4HIK
Crystal Structure of Schizosaccharomyces pombe Pot1pC bound to ssDNA (GGTTACGGT)
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-10-05 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9799, 0.9801, 0.9428 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.870, 57.390, 66.170 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.348 - 1.636 |
R-factor | 0.1947 |
Rwork | 0.194 |
R-free | 0.21570 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.058 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SnB |
Refinement software | PHENIX (1.7_650) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.670 |
High resolution limit [Å] | 1.636 | 4.450 | 1.640 |
Rmerge | 0.041 | 0.024 | 0.134 |
<I/σ(I)> | 26.4 | ||
Completeness [%] | 99.1 | 98.5 | 93.8 |
Redundancy | 4.5 | 4.6 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 290 | 25% w/V PEG 4000, 0.2M ammonium formate, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 290K |