4HIH
Anti-Streptococcus pneumoniae 23F Fab 023.102 with bound rhamnose.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-02-18 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.790, 66.680, 109.280 |
| Unit cell angles | 90.00, 101.28, 90.00 |
Refinement procedure
| Resolution | 17.000 - 2.000 |
| R-factor | 0.224 |
| Rwork | 0.224 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hie |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.582 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.000 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.053 | 0.286 |
| Number of reflections | 69510 | |
| <I/σ(I)> | 17.86 | 2.94 |
| Completeness [%] | 98.3 | 86.6 |
| Redundancy | 1.81 | 1.43 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 298 | 0.05M Na Citrate, pH 3-4, 18% PEG 3350, 0.2M Li Acetate , VAPOR DIFFUSION, HANGING DROP, temperature 298K |
