4HI8
Structure of integrin-linked kinase ankyrin repeat domain in complex with PINCH1 LIM1 domain collected at high energy, wavelength 0.32800
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | OTHER |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-11-13 |
| Detector | MAR scanner 180 mm plate |
| Wavelength(s) | 0.32800 |
| Spacegroup name | P 1 |
| Unit cell lengths | 37.093, 41.859, 46.983 |
| Unit cell angles | 79.87, 68.82, 85.77 |
Refinement procedure
| Resolution | 17.786 - 1.203 |
| R-factor | 0.1449 |
| Rwork | 0.144 |
| R-free | 0.16820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3f6q |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.602 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.3.0) |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.240 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.057 | 0.582 |
| Number of reflections | 79561 | |
| <I/σ(I)> | 7.4 | 1.275 |
| Completeness [%] | 99.3 | 99.1 |
| Redundancy | 2.2 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 296 | 20% PEG 3350, 0.2M Sodium formate, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
