4HI5
crystal structure of F37A mutant of borna disease virus matrix protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-07-10 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | I 4 3 2 |
Unit cell lengths | 139.637, 139.637, 139.637 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 70.000 - 3.600 |
R-factor | 0.2477 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3f1j |
RMSD bond length | 0.006 |
RMSD bond angle | 1.008 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 70.000 |
High resolution limit [Å] | 3.600 |
Rmerge | 0.134 |
Number of reflections | 2918 |
<I/σ(I)> | 13.4 |
Completeness [%] | 100.0 |
Redundancy | 11 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.1 | 293 | 0.2 M MgCl2, 30% w/v PEG400, 0.1 M HEPES, pH 7.1, VAPOR DIFFUSION, HANGING DROP, temperature 293K |