4HHV
Crystal structure of ceramide transfer protein pleckstrin homology domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-03-23 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.131, 54.979, 98.988 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.490 - 1.750 |
R-factor | 0.1799 |
Rwork | 0.179 |
R-free | 0.20360 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.016 |
RMSD bond angle | 1.672 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.810 |
High resolution limit [Å] | 1.750 | 3.770 | 1.750 |
Rmerge | 0.069 | 0.054 | 0.378 |
Number of reflections | 27044 | ||
<I/σ(I)> | 13.8 | ||
Completeness [%] | 99.6 | 99.7 | 96.1 |
Redundancy | 13.9 | 13.7 | 10.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.1 M sodium citrate, pH 6.0, 1.0 M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |