4HHT
T. maritima RNase H2 G21S in complex with nucleic acid substrate and calcium ions
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-10-09 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.91841 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 106.952, 47.626, 77.451 |
Unit cell angles | 90.00, 131.26, 90.00 |
Refinement procedure
Resolution | 26.015 - 3.100 |
R-factor | 0.1818 |
Rwork | 0.178 |
R-free | 0.25420 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.255 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 29.109 |
High resolution limit [Å] | 3.100 |
Number of reflections | 5292 |
Completeness [%] | 96.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6 | 291 | 50 mM CaCl2, 0.1 M MES pH 6.0, 45% PEG200, VAPOR DIFFUSION, temperature 291K |