4HHS
Crystal Structure of fatty acid alpha-dioxygenase (Arabidopsis thaliana)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-13 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 102.150, 102.150, 137.565 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.700 |
| R-factor | 0.16054 |
| Rwork | 0.159 |
| R-free | 0.18733 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.207 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 102.150 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.071 | 0.558 |
| Number of reflections | 79943 | |
| <I/σ(I)> | 39.4 | 5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.4 | 12.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 300 | 28% PEG400, 50mM Tris, 0.2M calcium chloride, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 300K |
