4HHR
Crystal Structure of fatty acid alpha-dioxygenase (Arabidopsis thaliana)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2010-03-24 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 1.03319 |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 101.935, 101.935, 137.510 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.780 - 1.510 |
R-factor | 0.15403 |
Rwork | 0.153 |
R-free | 0.18227 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 1.427 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXS |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.540 |
High resolution limit [Å] | 1.510 | 1.510 |
Rmerge | 0.067 | 0.527 |
Number of reflections | 107022 | |
<I/σ(I)> | 28.4 | 3 |
Completeness [%] | 94.2 | 93.5 |
Redundancy | 8.3 | 7.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 300 | 28% PEG400, 50mM Tris, 0.2M calcium chloride, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 300K |