4HHL
High resolution crystal structure of Glucose Isomerase from Streptomyces sp. SK
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-11-13 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.97946 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 110.800, 91.680, 84.610 |
Unit cell angles | 90.00, 119.65, 90.00 |
Refinement procedure
Resolution | 48.140 - 1.730 |
R-factor | 0.13378 |
Rwork | 0.131 |
R-free | 0.18786 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1muw |
RMSD bond length | 0.024 |
RMSD bond angle | 1.795 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | BALBES |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.140 | 1.900 |
High resolution limit [Å] | 1.730 | 1.730 |
Number of reflections | 74395 | |
<I/σ(I)> | 13 | |
Completeness [%] | 96.4 | 93.4 |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 298 | PEG 3350, Mg acetate, pH 7, VAPOR DIFFUSION, temperature 298.0K |