4HH8

Crystal structure of bovine butyrophilin

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-12-15
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97973,0.97985,0.96863,0.99188
Spacegroup name	P 21 21 2
Unit cell lengths	73.180, 101.020, 44.310
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	59.260 - 2.300
R-factor	0.2282
Rwork	0.226
R-free	0.27840
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	1.630
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.9)
Phasing software	Auto-Rickshaw
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	59.264	37.903	2.420
High resolution limit [Å]	2.300	7.270	2.300
Rmerge		0.025	0.301
Total number of observations		3215	15391
Number of reflections	15058
<I/σ(I)>	16.7	24.5	2.5
Completeness [%]	99.3	96.9	99.1
Redundancy	7.1	6.1	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	293	1.5 M potassium phosphate, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K