4HH8
Crystal structure of bovine butyrophilin
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97973,0.97985,0.96863,0.99188 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 73.180, 101.020, 44.310 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 59.260 - 2.300 |
| R-factor | 0.2282 |
| Rwork | 0.226 |
| R-free | 0.27840 |
| Structure solution method | MAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.630 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.9) |
| Phasing software | Auto-Rickshaw |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 59.264 | 37.903 | 2.420 |
| High resolution limit [Å] | 2.300 | 7.270 | 2.300 |
| Rmerge | 0.025 | 0.301 | |
| Total number of observations | 3215 | 15391 | |
| Number of reflections | 15058 | ||
| <I/σ(I)> | 16.7 | 24.5 | 2.5 |
| Completeness [%] | 99.3 | 96.9 | 99.1 |
| Redundancy | 7.1 | 6.1 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 1.5 M potassium phosphate, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
