4HH3
Structure of the AppA-PpsR2 core complex from Rb. sphaeroides
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-08-12 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.99981 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 89.780, 188.300, 51.860 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.907 - 1.750 |
R-factor | 0.1713 |
Rwork | 0.170 |
R-free | 0.19800 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.022 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.850 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.005 | 0.684 |
Number of reflections | 89594 | |
<I/σ(I)> | 20.3 | 2.85 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 8.6 | 8.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.1M HEPES, 64 mM tri-sodium citrate, 10% PEG 5000 monomethyl ether, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |