4HH2
Structure of PpsR without the HTH motif from Rb. sphaeroides
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-12-02 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9483 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 117.450, 117.450, 211.820 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.971 - 2.800 |
| R-factor | 0.1841 |
| Rwork | 0.181 |
| R-free | 0.23480 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.842 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX-AutoSol |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.043 | 0.681 |
| Number of reflections | 41955 | |
| <I/σ(I)> | 17.4 | 2.7 |
| Completeness [%] | 99.0 | 99.6 |
| Redundancy | 5.2 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1M tris chloride buffer, 1.9M ammonium sulfate, 12% glycerol, 6% xylitol, 1% dioxane, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
