4HH0
Dark-state structure of AppA C20S without the Cys-rich region from Rb. sphaeroides
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-06-22 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 32 1 2 |
Unit cell lengths | 70.260, 70.260, 382.100 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.601 - 2.600 |
R-factor | 0.187 |
Rwork | 0.185 |
R-free | 0.22670 |
Structure solution method | native data for SAD phase extension |
RMSD bond length | 0.005 |
RMSD bond angle | 0.870 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX-AutoSol |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.000 | 2.700 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.052 | 0.629 |
Number of reflections | 33847 | |
<I/σ(I)> | 18.2 | 2.63 |
Completeness [%] | 99.7 | 99.8 |
Redundancy | 5.5 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 0.3M tris chloride buffer, 1.3M sodium chloride, 0.3M magnesium chloride, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |