4HGU
Crystal Structure of Galleria mellonella Silk Protease Inhibitor 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-06-05 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.80000,0.91841 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 27.270, 31.240, 35.740 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 0.980 |
R-factor | 0.1057 |
Rwork | 0.106 |
R-free | 0.12360 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1an1 |
RMSD bond length | 0.023 |
RMSD bond angle | 0.058 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.010 |
High resolution limit [Å] | 0.980 | 0.980 |
Rmerge | 0.072 | 0.460 |
Number of reflections | 17179 | |
<I/σ(I)> | 22.52 | 2.04 |
Completeness [%] | 94.8 | 84.5 |
Redundancy | 12.9 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 292 | 1.4 M sodium citrate and 0.1 M Hepes pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |