4HGA
Structure of the variant histone H3.3-H4 heterodimer in complex with its chaperone DAXX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.070 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 72.103, 72.103, 323.550 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.934 - 2.799 |
| R-factor | 0.2105 |
| Rwork | 0.208 |
| R-free | 0.27080 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.905 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.799 | 2.800 |
| Rmerge | 0.083 | 0.521 |
| Number of reflections | 13274 | |
| <I/σ(I)> | 21.9 | 1.91 |
| Completeness [%] | 99.8 | 99.1 |
| Redundancy | 7.8 | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 298 | 0.1M HEPES-Na, pH 7.6, 23% (v/v) PEG 3350, 0.25M Ammonium acetate, 1% Tacsimate pH 7.0, 6% ethanol, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
