4HG6

Structure of a cellulose synthase - cellulose translocation intermediate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2012-03-04
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97949
Spacegroup name	P 43 21 2
Unit cell lengths	103.120, 103.120, 468.320
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.810 - 3.250
R-factor	0.21622
Rwork	0.213
R-free	0.28172
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.369
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHELXS
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.420
High resolution limit [Å]	2.800	3.250
Number of reflections	41133
Completeness [%]	99.9	99

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	277	30% PEG200, 50mM NaCl, 0.1M MES, pH 6.5, VAPOR DIFFUSION, temperature 277K