4HG5
Structural insights into yeast Nit2: wild-type yeast Nit2 in complex with oxaloacetate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-26 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97917 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.498, 125.840, 77.879 |
| Unit cell angles | 90.00, 95.41, 90.00 |
Refinement procedure
| Resolution | 32.750 - 1.910 |
| R-factor | 0.20141 |
| Rwork | 0.200 |
| R-free | 0.22882 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4h5u |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.063 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.940 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Rmerge | 0.817 | |
| Number of reflections | 96835 | |
| <I/σ(I)> | 3.13 | |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 287 | 17.5% PEG4000, 0.1M sodium cacodylate, 100mM sodium oxaloacetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 287K |
