4HG0

Crystal Structure of magnesium and cobalt efflux protein CorC, Northeast Structural Genomics Consortium (NESG) Target ER40

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2011-09-14
Detector	ADSC QUANTUM 4
Wavelength(s)	0.979
Spacegroup name	P 32 2 1
Unit cell lengths	71.953, 71.953, 123.530
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.818 - 3.102
R-factor	0.2147
Rwork	0.211
R-free	0.28010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3nqr
RMSD bond length	0.010
RMSD bond angle	1.391
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	BALBES
Refinement software	PHENIX (dev_1269)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	3.100
Rmerge	0.059
Number of reflections	7056
<I/σ(I)>	28.2
Completeness [%]	97.7
Redundancy	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	microbatch under oil	7	293	Protein solution: 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl (pH 7.5), Reservoir solution:0.2M Na-malonate, 20% PEG 3300, microbatch under oil, temperature 293K