Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0441 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.083, 48.492, 112.347 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.730 - 2.000 |
| R-factor | 0.1888 |
| Rwork | 0.185 |
| R-free | 0.25760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4flb |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.407 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0027) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 40.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 5.420 | 2.000 |
| Rmerge | 0.103 | 0.032 | 0.885 |
| Number of reflections | 18559 | ||
| <I/σ(I)> | 7.6 | ||
| Completeness [%] | 99.8 | 99.9 | 97.6 |
| Redundancy | 6.9 | 6.5 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 291 | 30% PEG550MME, 0.1M ammounium sulfate, 0.1M sodium cacodylate, pH 6.5, vapor diffusion, temperature 291K |
