4HFF
Crystal structure of the type VI effector-immunity complex Tae4-Tai4 from Salmonella Typhimurium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BSRF BEAMLINE 3W1A |
| Synchrotron site | BSRF |
| Beamline | 3W1A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-15 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 63.903, 63.903, 365.916 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.001 - 2.398 |
| R-factor | 0.1884 |
| Rwork | 0.186 |
| R-free | 0.22560 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.064 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.440 |
| High resolution limit [Å] | 2.398 | 2.400 |
| Rmerge | 0.082 | 0.433 |
| Number of reflections | 18525 | |
| <I/σ(I)> | 38.1 | 4 |
| Completeness [%] | 100.0 | 94.3 |
| Redundancy | 17.6 | 12.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 0.2M ammonium acetate, 0.1M sodium citrate tribasic dihydrate (pH 5.6), 30% v/v (+/-)-2-methyl-2,4-pentanedio, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
