4HF0
Crystal Structure of Apo IscR
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-09-15 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.97 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 88.850, 88.850, 62.734 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.622 - 1.900 |
R-factor | 0.1957 |
Rwork | 0.193 |
R-free | 0.22050 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 0.984 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.1_1161) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.900 |
Number of reflections | 22775 |
Completeness [%] | 100.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 2M Ammonium Sulfate, 20% glycerol, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |