4HEM
Llama vHH-02 binder of ORF49 (RBP) from lactococcal phage TP901-1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-09-19 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.931 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 70.980, 104.250, 122.180 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.160 - 1.650 |
R-factor | 0.1677 |
Rwork | 0.167 |
R-free | 0.17720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3hg0 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.090 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | BUSTER (2.11.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.000 | 1.690 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.054 | 0.530 |
Number of reflections | 108049 | |
<I/σ(I)> | 26 | 5.7 |
Completeness [%] | 90.7 | 97.5 |
Redundancy | 15 | 15 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | 30% PEG 4000, 0.2M ammonium sulfate, 0.1M Na acetate, 4.7 mg/ml Protein complex , pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K |