4HEB
The Crystal structure of Maf protein of Bacillus subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-09-22 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.550, 85.950, 95.030 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.740 - 2.260 |
| R-factor | 0.2155 |
| Rwork | 0.215 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ex2 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP (10.2..35) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.300 |
| High resolution limit [Å] | 2.260 | 2.260 |
| Number of reflections | 24272 | |
| <I/σ(I)> | 19.7 | |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 7.3 | 0.884 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 15% PEG 3500, 0.1 M succinate acid pH7.0, vapor diffusion hanging drop, temperature 295K |
