4HE7
Crystal Structure of Brazzein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 293 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1995-01-01 |
| Detector | WEISSENBERG |
| Wavelength(s) | 1.04 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 61.400, 61.400, 59.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.800 - 1.800 |
| R-factor | 0.21933 |
| Rwork | 0.218 |
| R-free | 0.25162 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2brz |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.124 |
| Data reduction software | HKL-2000 |
| Data scaling software | WEIS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.800 | 1.847 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 6728 | |
| Completeness [%] | 89.4 | 83.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293 | 0.9-1.0M sodium citrate, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
