4HE4
Crystal structure of the yellow fluorescent protein phiYFP (Phialidium sp.)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 102.870, 102.870, 242.530 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.700 - 2.050 |
| R-factor | 0.1989 |
| Rwork | 0.196 |
| R-free | 0.25570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qy3 |
| RMSD bond length | 0.026 |
| RMSD bond angle | 1.872 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.120 |
| High resolution limit [Å] | 2.050 | 4.410 | 2.050 |
| Rmerge | 0.078 | 0.044 | 0.562 |
| Number of reflections | 31372 | ||
| <I/σ(I)> | 11 | ||
| Completeness [%] | 99.7 | 99.8 | 98.2 |
| Redundancy | 7.6 | 7.4 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.16 M NaH2PO4 x 2H2O, 16% PEG 3350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
