4HE1
Crystal structure of human muscle fructose-1,6-bisphosphatase Q32R mutant complex with fructose-6-phosphate and phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-18 |
| Detector | SBC-3 |
| Wavelength(s) | 0.9000 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 73.818, 73.818, 146.575 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.230 |
| R-factor | 0.1939 |
| Rwork | 0.193 |
| R-free | 0.21130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1eyi |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.210 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.310 |
| High resolution limit [Å] | 2.230 | 4.800 | 2.230 |
| Rmerge | 0.104 | 0.044 | 0.500 |
| Number of reflections | 20427 | ||
| <I/σ(I)> | 10 | ||
| Completeness [%] | 99.5 | 95.3 | 100 |
| Redundancy | 13.7 | 11.9 | 14.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 0.1M MgCl2, 15% PEG 4000, 0.1M Hepes pH 7.5, vapor diffusion, hanging drop, temperature 295K |
