4HDO
Crystal structure of the binary Complex of KRIT1 bound to the Rap1 GTPase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-07-23 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 57.850, 77.750, 58.850 |
Unit cell angles | 90.00, 91.20, 90.00 |
Refinement procedure
Resolution | 32.430 - 1.670 |
R-factor | 0.21253 |
Rwork | 0.212 |
R-free | 0.23142 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1c1y |
RMSD bond length | 0.005 |
RMSD bond angle | 1.006 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.770 |
High resolution limit [Å] | 1.670 | 1.670 |
Rmerge | 0.046 | 0.350 |
Number of reflections | 57913 | |
<I/σ(I)> | 31.27 | 4.37 |
Completeness [%] | 96.0 | 80 |
Redundancy | 3.6 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 278 | 15% PEG2000 MME, 100 mM Tris, 100 mM potassium chloride, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 278K |