4HDH
Crystal Structure of viral RdRp in complex with ATP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-11-27 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 144.838, 86.506, 112.805 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 144.838 - 2.280 |
R-factor | 0.22796 |
Rwork | 0.225 |
R-free | 0.27151 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2hfz |
RMSD bond length | 0.018 |
RMSD bond angle | 1.620 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 144.838 | 2.339 |
High resolution limit [Å] | 2.280 | 2.280 |
Rmerge | 0.070 | 0.435 |
Number of reflections | 65396 | |
<I/σ(I)> | 21.2 | 2.8 |
Completeness [%] | 99.4 | 97.4 |
Redundancy | 5 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |