4HDH
Crystal Structure of viral RdRp in complex with ATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-27 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 144.838, 86.506, 112.805 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 144.838 - 2.280 |
| R-factor | 0.22796 |
| Rwork | 0.225 |
| R-free | 0.27151 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hfz |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.620 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 144.838 | 2.339 |
| High resolution limit [Å] | 2.280 | 2.280 |
| Rmerge | 0.070 | 0.435 |
| Number of reflections | 65396 | |
| <I/σ(I)> | 21.2 | 2.8 |
| Completeness [%] | 99.4 | 97.4 |
| Redundancy | 5 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
