4HD1
Crystal structure of squalene synthase HpnC from Alicyclobacillus acidocaldarius
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-08-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97935 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 124.803, 124.803, 95.042 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.690 - 2.400 |
| R-factor | 0.2004 |
| Rwork | 0.198 |
| R-free | 0.24700 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.046 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.420 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.074 | 0.985 |
| Number of reflections | 17122 | |
| <I/σ(I)> | 49.5 | 2.7 |
| Completeness [%] | 97.3 | 14.3 |
| Redundancy | 14.4 | 14.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 0.2M Sodium Fluoride, 20% PEG3350, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
