4HCZ
PHF1 Tudor in complex with H3K36me3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-01-20 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.541 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 88.030, 29.670, 62.000 |
| Unit cell angles | 90.00, 116.41, 90.00 |
Refinement procedure
| Resolution | 23.413 - 1.850 |
| R-factor | 0.2058 |
| Rwork | 0.204 |
| R-free | 0.24390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2e5p |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.060 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.413 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 12091 | |
| Completeness [%] | 96.4 | 81.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277.15 | 0.1 M HEPES pH 7.5, 20% PEG 10000, VAPOR DIFFUSION, SITTING DROP, temperature 277.15K |
