4HCI
Uncharacterized Cupredoxin-like Domain Protein Cupredoxin_1 from Bacillus anthracis
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-08-13 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.97926 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 95.200, 42.809, 51.245 |
| Unit cell angles | 90.00, 120.40, 90.00 |
Refinement procedure
| Resolution | 24.521 - 1.630 |
| R-factor | 0.179 |
| Rwork | 0.178 |
| R-free | 0.20200 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.132 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1161)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.630 | 1.630 |
| Number of reflections | 21617 | |
| <I/σ(I)> | 19.9 | 0.508 |
| Completeness [%] | 96.8 | 93.2 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 289 | 2.86 M ammonium nitrate, 150 mM Tris pH 8.0, 100 mM sodium iodide, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
