4HCB
The metal-free form of crystal structure of E.coli ExoI-ssDNA complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-06-22 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0720 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 62.544, 107.289, 157.574 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.663 - 2.000 |
R-factor | 0.1849 |
Rwork | 0.183 |
R-free | 0.22440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3c95 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.002 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASES |
Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
Rmerge | 0.083 | 0.052 | 0.297 |
Number of reflections | 71457 | ||
<I/σ(I)> | 14.2 | ||
Completeness [%] | 99.1 | 95.7 | 99.7 |
Redundancy | 8.3 | 7.8 | 8.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 1.65-1.68 M ammonium sulfate, 6.5-6.8% v/v 2-propanol, 0.1 M sodium citrate tribasic dihydrate pH 6.0 and 5 mM phenol, VAPOR DIFFUSION, SITTING DROP, temperature 293K |