4HC5
Crystal structure of member of Glyoxalase/bleomycin resistance protein/dioxygenase superfamily from Sphaerobacter thermophilus DSM 20745
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-01-08 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9794 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 105.883, 71.023, 86.698 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.590 - 1.450 |
R-factor | 0.1391 |
Rwork | 0.137 |
R-free | 0.18271 |
Structure solution method | SAD |
RMSD bond length | 0.023 |
RMSD bond angle | 2.031 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 86.000 | 1.480 |
High resolution limit [Å] | 1.450 | 1.450 |
Rmerge | 0.085 | |
Number of reflections | 115474 | |
<I/σ(I)> | 40 | |
Completeness [%] | 99.0 | 99.9 |
Redundancy | 11.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | Ammonium sulfate 1.5 M, Tris 0.1 M, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |