4HB5
Crystal Structure of Engineered Protein. Northeast Structural Genomics Consortium Target OR267.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-09-21 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97885 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.977, 45.469, 63.152 |
| Unit cell angles | 90.00, 107.17, 90.00 |
Refinement procedure
| Resolution | 35.537 - 2.294 |
| R-factor | 0.196 |
| Rwork | 0.194 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4gpm |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.375 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.370 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.071 | 0.146 |
| Number of reflections | 26798 | |
| <I/σ(I)> | 24.5 | 8.8 |
| Completeness [%] | 98.9 | 99.9 |
| Redundancy | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microbatch crystallization under oil | 8 | 277 | 20% PEG 4000, 0.1M magnesium sulfate, 0.1M Tris-HCl, pH 8.0, Microbatch crystallization under oil, temperature 277K |
