4HAH
Structure of the Salmonella plasmid virulence C protein (SpvC)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 80 |
Detector technology | IMAGE PLATE |
Collection date | 2011-05-20 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 64.528, 71.912, 106.490 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.264 - 1.800 |
R-factor | 0.1952 |
Rwork | 0.194 |
R-free | 0.23100 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.024 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.264 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.107 | 0.485 |
Number of reflections | 45887 | |
<I/σ(I)> | 9 | 2.5 |
Completeness [%] | 98.4 | 86.8 |
Redundancy | 7.22 | 4.35 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 20% PEG 3350 (a 50% w/v solution) 0.1M Ammonium Chloride (1M stock solution) , pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |