4HAF

Crystal structure of fc-fragment of human IgG2 antibody (primitive crystal form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2010-03-19
Detector	RIGAKU SATURN 944
Spacegroup name	P 21 21 21
Unit cell lengths	49.500, 75.170, 146.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.040
R-factor	0.20041
Rwork	0.199
R-free	0.24819
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ave
RMSD bond length	0.009
RMSD bond angle	1.189
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.700	2.090
High resolution limit [Å]	2.040	2.040
Rmerge	0.085	0.357
Number of reflections	35162
<I/σ(I)>	18.7	3.1
Completeness [%]	98.7	83.9
Redundancy	6.7	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.1 M MES, 9% PEG 4000, 0.2 M AMMONIUM CHLORIDE, 5% MPD CRYO CONDITIONS: 0.1 M MES, 12% PEG 4000, 0.2 M AMMONIUM CHLORIDE, 20% PEG 400, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K