4HAA
Structure of Ribonuclease Binase Glu43Ala/Phe81Ala Mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | KURCHATOV SNC BEAMLINE K4.4 |
Synchrotron site | KURCHATOV SNC |
Beamline | K4.4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-11-10 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.983 |
Spacegroup name | P 43 |
Unit cell lengths | 50.360, 50.360, 196.120 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 18.730 - 1.900 |
R-factor | 0.1786 |
Rwork | 0.177 |
R-free | 0.20544 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gov |
RMSD bond length | 0.017 |
RMSD bond angle | 1.782 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.000 | 2.010 |
High resolution limit [Å] | 1.890 | 1.890 |
Rmerge | 0.091 | 0.492 |
Number of reflections | 37967 | |
<I/σ(I)> | 2.49 | |
Completeness [%] | 97.7 | 89.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 3.5 | 298 | 10 mg/mL protein, reservoir: 0.1 M citric acid, pH 3.5, 3 M sodium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 298K |