4HA8
Structure of Staphylococcus aureus biotin protein ligase in complex with biotin acetylene
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 93.020, 93.020, 129.940 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.810 - 2.650 |
| R-factor | 0.19889 |
| Rwork | 0.197 |
| R-free | 0.23594 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.865 |
| Data scaling software | XDS |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.650 |
| Number of reflections | 16247 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 10% PEG 8000, 0.1M Tris pH 7.5, 10% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
