4HA7
Structural insights into the reduction mechanism of Saccharomyces cerevisia Riboflavin Biosynthesis Reductase Rib7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-04-11 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9793 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.500, 68.623, 148.954 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.749 - 2.100 |
R-factor | 0.203 |
Rwork | 0.200 |
R-free | 0.26610 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.235 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.750 | 2.140 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 27560 | |
Completeness [%] | 98.5 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 0.05M Calcium chloride dihydrate, 0.1M BIS-TRIS, 30% v/v Polyethylene glycol monomethyl ether 550 , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |