4HA4
Structure of beta-glycosidase from Acidilobus saccharovorans in complex with glycerol
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | KURCHATOV SNC BEAMLINE K4.4 |
Synchrotron site | KURCHATOV SNC |
Beamline | K4.4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-06-01 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.9815 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 84.120, 84.120, 166.270 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.827 - 1.370 |
R-factor | 0.13213 |
Rwork | 0.130 |
R-free | 0.16333 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 1.559 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | BALBES |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.827 | 1.450 |
High resolution limit [Å] | 1.370 | 1.370 |
Rmerge | 0.063 | 0.810 |
Number of reflections | 124046 | |
<I/σ(I)> | 17.2 | 2.1 |
Completeness [%] | 98.8 | 96.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Protein solution (1.5mcl): 13mg/ml Asbeta-Gly, 0.025M NaCl, 0.025M Hepes (pH 7.5). Reservoir solution (1.5mcl): 1.0M succinic acid (pH 7.0), 0.1M Hepes (pH 7.0), 1% w/v polyethylene glycol monomethyl ether 2000. The crystal was soaked in a cryoprotection solution containing 20% v/v glycerol., VAPOR DIFFUSION, HANGING DROP, temperature 293K |