4H9Q

Complex structure 4 of DAXX(E225A)/H3.3(sub5)/H4

Experimental procedure

Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Wavelength(s)	0.979
Spacegroup name	P 43 21 2
Unit cell lengths	107.522, 107.522, 90.463
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.873 - 1.950
R-factor	0.1924
Rwork	0.191
R-free	0.22090
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.965
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.2_869))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.950
Number of reflections	39075
Completeness [%]	99.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277	1.8 M Na/K-phosphate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K