4H8X

Radiation damage study of lysozyme - 0.07 MGy

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-02-12
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	1.0332
Spacegroup name	P 43 21 2
Unit cell lengths	78.767, 78.767, 36.866
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.914 - 1.200
R-factor	0.1913
Rwork	0.191
R-free	0.19970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6lyz
RMSD bond length	0.007
RMSD bond angle	1.109
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX (1.7.2_862)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.220
High resolution limit [Å]	1.200	3.260	1.200
Rmerge	0.032	0.018	0.300
Number of reflections	36858
<I/σ(I)>	45.1
Completeness [%]	99.3	93	100
Redundancy	4.5	4.4	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.8	298	7.5% sodium chloride, 100 mM sodium acetate buffer and 25% ethylene glycol, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K