4H8M
Crystal structure of a parallel 6-helix coiled coil CC-Hex-H24-A5/7C
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Detector technology | CCD |
Collection date | 2011-07-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97 |
Spacegroup name | H 3 |
Unit cell lengths | 31.700, 31.700, 132.040 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.013 - 1.429 |
R-factor | 0.1973 |
Rwork | 0.197 |
R-free | 0.20350 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3r46 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.573 |
Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 44.013 |
High resolution limit [Å] | 1.429 |
Number of reflections | 9078 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 30 mM sodium fluoride, 30 mM sodium bromide, 30 mM sodium iodide, 50 mM sodium HEPES, 50 mM MOPS (acid) pH 7.5, 20 % PEG-MME 550 and 10 % PEG 20K, VAPOR DIFFUSION, SITTING DROP, temperature 291K |