4H8H
MUTB inactive double mutant E254Q-D415N
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-22 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.93300 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.930, 84.410, 118.970 |
| Unit cell angles | 90.00, 95.94, 90.00 |
Refinement procedure
| Resolution | 44.900 - 2.000 |
| R-factor | 0.1831 |
| Rwork | 0.181 |
| R-free | 0.22780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zjb |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.861 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.900 | 2.200 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 83234 | |
| <I/σ(I)> | 13.34 | 3.4 |
| Completeness [%] | 99.4 | 98 |
| Redundancy | 4.2 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 292 | 0.2M AMMONIUM SULFATE, 20% PEG3350, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
