4H8F

Crystal structure of a parallel 4-helix coiled coil CC-Hex-II- 22

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Detector technology	PIXEL
Collection date	2011-08-22
Detector	PSI PILATUS 6M
Wavelength(s)	0.97
Spacegroup name	P 41 2 2
Unit cell lengths	46.670, 46.670, 94.060
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.670 - 1.880
R-factor	0.20878
Rwork	0.207
R-free	0.24324
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3r46
RMSD bond length	0.014
RMSD bond angle	1.241
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall
Low resolution limit [Å]	46.700
High resolution limit [Å]	1.880
Number of reflections	8847

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	25 % tert-butanol and 100 mM Tris pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	25 % tert-butanol and 100 mM Tris pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K