4H89
The Structure of a GCN5-Related N-Acetyltransferase from Kribbella flavida
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-07-08 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97931, 0.97945 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 42.482, 64.913, 56.569 |
Unit cell angles | 90.00, 100.68, 90.00 |
Refinement procedure
Resolution | 18.450 - 1.370 |
R-factor | 0.1196 |
Rwork | 0.117 |
R-free | 0.15870 |
Structure solution method | MAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.689 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | HKL-3000 |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.390 |
High resolution limit [Å] | 1.370 | 3.720 | 1.370 |
Rmerge | 0.100 | 0.092 | 0.351 |
Number of reflections | 30480 | ||
<I/σ(I)> | 10.9 | ||
Completeness [%] | 95.8 | 88.6 | 92.5 |
Redundancy | 4.6 | 4.4 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 297 | 0.1M HEPES:NaOH pH 7.5, 1.4M Sodium Citrate, VAPOR DIFFUSION, SITTING DROP, temperature 297K |