4H87

Crystal structure of a FHA domain of kanadaptin (SLC4A1AP) from Homo sapiens at 1.55 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-08-01
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 21 21 21
Unit cell lengths	34.768, 82.141, 82.467
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.764 - 1.550
R-factor	0.1767
Rwork	0.175
R-free	0.19830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3els
RMSD bond length	0.010
RMSD bond angle	1.010
Data reduction software	XDS
Data scaling software	XSCALE (March 15, 2012)
Phasing software	MOLREP (11.0.02)
Refinement software	BUSTER-TNT (2.10.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	36.764	36.764	1.610
High resolution limit [Å]	1.550	3.330	1.550
Rmerge	0.048	0.021	0.885
Number of reflections	35157	3781	3706
<I/σ(I)>	27.74	80.6	2.9
Completeness [%]	99.9	99.5	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	277	1.60M ammonium sulfate, 0.1M citric acid pH 5.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K