4H86
Crystal structure of Ahp1 from Saccharomyces cerevisiae in reduced form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-11-08 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.97915 |
Spacegroup name | I 21 3 |
Unit cell lengths | 149.049, 149.049, 149.049 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.131 - 2.004 |
R-factor | 0.1584 |
Rwork | 0.158 |
R-free | 0.17340 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 1TP9 3uma 1NM3 AND 2PWJ |
RMSD bond length | 0.006 |
RMSD bond angle | 0.907 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.030 |
High resolution limit [Å] | 2.000 | 5.430 | 2.000 |
Rmerge | 0.125 | 0.076 | 0.765 |
Number of reflections | 37056 | ||
<I/σ(I)> | 9 | ||
Completeness [%] | 100.0 | 99.7 | 100 |
Redundancy | 44.8 | 43.4 | 45 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 298 | Protein Solution (40 mg/ml Ahp1 protein, in Milli-Q water containing 10mM DTT) mixed in a 1:1 ratio with the well solution (0.1 M BIS-TRIS pH 5.4, 2.0 M Ammonium Sulfate) Cryoprotected with 20% Glycerol , VAPOR DIFFUSION, HANGING DROP, temperature 298K |