4H7H

Crystal structure of haloalkane dehalogenase LinB T135A mutant from Sphingobium sp. MI1205

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Collection date	2010-03-02
Wavelength(s)	1.0000
Spacegroup name	P 21 21 2
Unit cell lengths	50.353, 71.711, 73.072
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.100
R-factor	0.18864
Rwork	0.186
R-free	0.25014
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4h77
RMSD bond length	0.009
RMSD bond angle	1.371
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.100
Number of reflections	16096

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		278	VAPOR DIFFUSION, SITTING DROP, temperature 278K