4H7E

Crystal structure of haloalkane dehalogenase LinB V112A mutant from Sphingobium sp. MI1205

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Collection date	2010-09-30
Wavelength(s)	1.0000
Spacegroup name	P 21 21 2
Unit cell lengths	50.427, 72.219, 73.911
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.800
R-factor	0.17503
Rwork	0.174
R-free	0.19805
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4h77
RMSD bond length	0.007
RMSD bond angle	1.160
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	1.800
Number of reflections	25428

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		278	VAPOR DIFFUSION, SITTING DROP, temperature 278K